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Istituto di Ricerche Farmalogiche "Mario Negri"

Istituto di Ricerche Farmalogiche "Mario Negri"


Dr. Gianluigi Forloni


Team Leader: Dr. Gianluigi Forloni
Via G. La Masa 19
Milano, 20156, Italy
Contact: Dr. Gianluigi Forloni
Tel: +39 (0) 239 01 44 62
Fax: +39 (0) 239 00 19 16
Institute website:

Main area of research

  • Biology and genetic of neurodegenerative research including TSE, AD and PD with the specific attention to the role of protein misfolding
  • Development of in vitro and in vivo models for the study of familial TSE: The role of Prion protein aggregation in the infectivity and in pathogenesis of TSE, studies in cell-free conditions (EM, AFM, CD) to evaluate the protein aggregation process and the identification of interfering substances
  • Therapeutic approaches to TSE using experimental scrapie and transgenic mice: Testing of anti-amyloidogenic drugs and development of stem cells strategy

Research Team Picture

Research Team

  • Researchers: 4
  • Technicians: 3
  • Postdocs: 4
  • PhD/MD Students: 4

Facility Picture

Facilities and Equipment

  • Laboratories: BSL1, BSL2, BSL3
  • General equipment: Cell Biology, Cell culture, Pathology, Neurobiology, Biochemistry, Molecular biology, Biophysics, Histology, Imaging
  • Special equipment: Two photons Microscope, MRI, Atomic Force Microscope, EM, Time lapse microscope, magnetic plasmon resonance apparatus (ProteOn, Biorad), dHPLC, Mass Spectotrometry for proteomic analysis
  • Specific methods: Pharmacological analysis, Proteomics, long synthetic peptide synthesis, Development of transgenic mice, chemico physical analysis

Main references

  • De Luigi A, Colombo L, Diomede L, Capobianco R, Mangieri M, Miccolo C, Limido L, Forloni G, Tagliavini F, Salmona M. (2008). The efficacy of tetracyclines in peripheral and intracerebral prion infection. PLoS ONE.; 3:e1888
  • Fioriti L, Angeretti N, Colombo L, De Luigi A, Colombo A, Manzoni C, Morbin M, Tagliavini F, Salmona M, Chiesa R, Forloni G. (2007) Neurotoxic and gliotrophic activity of a synthetic peptide homologous to Gerstmann-Sträussler-Scheinker disease amyloid protein. J Neurosci. 27:1576-83
  • Biasini E, Massignan T, Fioriti L, Rossi V, Dossena S, Salmona M, Forloni G, Bonetto V, Chiesa R. (2006) Analysis of the cerebellar proteome in a transgenic mouse model of inherited prion disease reveals preclinical alteration of calcineurin activity. Proteomics 6:2823-34
  • Forloni G, Iussich S, Awan T, Colombo L, Angeretti N, Girola L, Bertani I, Poli G, Caramelli M, Grazia Bruzzone M, Farina L, Limido L, Rossi G, Giaccone G, Ironside JW, Bugiani O, Salmona M, Tagliavini F. (2002) Tetracyclines affect prion infectivity. Proc Natl Acad Sci U S A. 99:10849-54
  • Forloni G, Angeretti N, Chiesa R, Monzani E, Salmona M, Bugiani O, Tagliavini F. (1993) Neurotoxicity of a prion protein fragment. Nature. 362: 543-6.

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