Università di Bologna
#21 - Dipartimento di Scienze Neurologiche
Contact
Team Leader: Dr. Piero Parchi
Via Ugo Foscolo 7
Bologna, 40123, Italy
Contact: Dr. Piero Parchi
Tel: +39 51 2092741
Fax: +39 51 2092751
E-mail: piero.parchi@unibo.it
Institute website:
http://www.eng.unibo.it/PortaleEn/default.htm
Main area of research
- Diagnosis: development of a highly sensitive diagnostic test for strain typing and early diagnosis of CJD
- Physiopathology: characterization of biochemical and biological properties of prion strains. Study of genetic risk factors in the development of CJD
- Neuropathology: characterization of neuropathological and molecular phenotypes of human prion disease
Research Team
- Researchers: 4
- Technicians: 2
- Postdocs: 1
- PhD/MD Students: 1
Facilities and Equipment
- Laboratories: BSL1, BSL2
- General equipment: Pathology, Neurobiology, Biochemistry, Chemistry, Molecular biology, Histology, Imaging
- Special equipment: 2D gel electrophoresis, automated sequencer, dHPLC
- Specific methods: Purification and detection techniques for abnormal forms of the prion protein in biological
- samples
Main references
- Notari S, Capellari S, Langeveld J, Giese A, Strammiello R, Gambetti P, Kretzschmar HA, Parchi P.(2007) A refined method for molecular typing reveals that co-occurrence of PrP(Sc) types in Creutzfeldt-Jakob disease is not the rule. Lab Invest 2007; 87: 1103-12.
- Capellari S, Cardone F, Notari S, Schininà ME, Marras B, Sita D, Baruzzi A, Pocchiari M, Parchi P. Creutzfeldt-Jakob disease associated with he R208H mutation in the prion protein gene. Neurology 2005; 64: 905-7
- Notari S, Capellari S, Giese A, Westner I, Baruzzi A, Ghetti B, Gambetti P, Kretzschmar HA, Parchi P. Effects of different experimental conditions on the PrPSc core generated by protease digestion: implications for strain typing and molecular classification of CJD. J Biol Chem 2004; 279:16797-804.
- Parchi P, Zou W, Wang W, Brown P, Capellari S, Ghetti B, Kopp N, Schulz-Schaeffer WJ, Kretzschmar HA, Head MW, Ironside JW, Gambetti P, Chen SG. Genetic influence on the structural variations of the abnormal prion protein. Proc Natl Acad Sci U S A 2000; 97:10168-72.
- Parchi P, Giese A, Capellari S, Brown P, Schulz-Schaeffer W, Windl O, Zerr I, Budka H, Kopp N, Piccardo P, Poser S, Rojiani A, Streichemberger N, Julien J, Vital C, Ghetti B, Gambetti P, Kretzschmar H. Classification of sporadic Creutzfeldt-Jakob disease based on molecular and phenotypic analysis of 300 subjects. Ann Neurol 1999; 46:224-33.
